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普諾賽9L/lacZ (大鼠膠質(zhì)肉瘤細胞)

品名: 普諾賽9L/lacZ (大鼠膠質(zhì)肉瘤細胞)
型號: CL-0637
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產(chǎn)品信息

名稱9L/lacZ (大鼠膠質(zhì)肉瘤細胞) (種屬鑒定正確)
別稱9L/LacZ
背景描述The cells constitutively express the lacZ reporter gene product, E. coli derived beta-gal, as revealed on tissue sections by histochemical stain, and single tumor cells can be identified. Lymphocytes and other responding cells can be identified by double labeling with antibodies on the same slide. The contrast between stained cells and background facilitates image analysis. The beta-gal expression is very stable, but cells may need to be re-cloned after months of growth in culture.
種屬大鼠
年齡(性別)雄性
組織來源腦,膠質(zhì)細胞
生長特性貼壁細胞
細胞形態(tài)成纖維細胞樣
細胞類型腫瘤細胞
腫瘤類型膠質(zhì)瘤細胞
生物安全等級1
生長培養(yǎng)基DMEM10%FBS1%P/S
推薦傳代比例1:4-1:8
推薦換液頻率2~3次/周
凍存條件凍存液:55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO溫度:液氮
培養(yǎng)條件氣相:空氣,95%;CO2,5%溫度:37℃
致瘤性Yes, forms tumors in the brains of CD Fischer 344 rats
基因表達情況beta galactosidase (beta-gal)
保藏機構(gòu)ATCC; CRL-2200


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